HSC Niche Biology and HSC Expansion Ex Vivo

HSC Niche Biology and HSC Expansion Ex Vivo.

Hematopoietic stem cell (HSC) transplantation can restore a new functional hematopoietic system in recipients in cases where the system of the recipient is not functional or for example is leukemic. However, the number of available donor HSCs is often too low for successful transplantation. Expansion of HSCs and thus HSC self-renewal ex vivo would greatly improve transplantation therapy in the ...

An intrinsic BM hematopoietic niche occupancy defect of HSC in scid mice facilitates exogenous HSC engraftment.

Although scid mice have been widely used for human HSC engraftment studies, the function of HSCs of scid mice has not been characterized. We hypothesized that the DNA repair defect of scid mice results in a stem cell defect that facilitates HSC engraftment. scid BM cells showed severely impaired repopulation potentials in the competitive repopulation assay. To assess the BM hematopoietic niche ...

Megakaryocytes promote murine osteoblastic HSC niche expansion and stem cell engraftment after radioablative conditioning.

Successful hematopoietic stem cell (HSC) transplantation requires donor HSC engraftment within specialized bone marrow microenvironments known as HSC niches. We have previously reported a profound remodeling of the endosteal osteoblastic HSC niche after total body irradiation (TBI), defined as relocalization of surviving megakaryocytes to the niche site and marked expansion of endosteal osteobl...

HSC niche: ample room for every guest stem cell.

Driving human–mouse avatars to understand the HSC niche

Adult stem cells hold immediate clinical applications, where hematopoietic stem cell (HSC) are the best-characterized somatic stem cell and are currently used in bone marrow (BM) transplantation (BMT). Human BMT, however, is still constrained by inefficiencies of achieving long-term hematopoietic reconstitution, which is thought to arise from an inability to expand HSCs ex vivo, and by the diff...